The yggH gene in Escherichia coli and ORF aq065 in the hyperthermophilic eubacterium Aquifex aeolicus have also been shown to encode tRNA (m 7 G) methyltransferases ( De Bie et al ., 2003; Okamoto et al ., 2004 ).
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The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase. J.Bacteriol. 185:3238-3243.
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The N7-methylguanosine (m 7 G) modification is common in bacterial and eukaryotic tRNAs and has been found in a few archaea (4). Most often, m 7 G is present at position 46 in the variable loop of tRNAs, although it has additionally been found at tRNA positions 34, 36, and 49 in a handful of organisms (4).
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We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N (7)-methylguanosine at position 46 (m (7)G46) in tRNA.
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The yggH Gene of Escherichia coli Encodes a tRNA (m7G46) Methyltransferase. The Escherichia coli YggH protein is cloned, expressed, and purified and it is shown that it catalyzes the S-adenosyl-L-methionine-dependent formation of N (7)-methylguanosine at position 46 (m (7)G46) in tRNA.
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The yggH ORF was PCR amplified from E. coli genomic DNA (strain XL1-Blue) by using Pfu DNA polymerase (Promega). The primers (Table 1 ) were designed to amplify the yggH ORF with its ribosome binding site.
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The 5′ cap of eukaryotic mRNA with N7 -methylguanosine (m 7 G) is an integral component for translation, splicing and mRNA export. m 7 G has been identified in mRNA and microRNA 4, 5. S...
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7-methylguanosine (m 7 G) modification of tRNA occurs widely in eukaryotes and bacteria, is nearly always found at position 46, and is one of the few modifications that confers a positive charge to the base.
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De Bie, L. G. et al. The yggH gene of Escherichia coli encodes a tRNA (m 7 G46) methyltransferase. J. Bacteriol. 185, 3238–3243 (2003).
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